jguarracino Posted August 13, 2015 Share Posted August 13, 2015 I suppose I should be glad, hahaha. I’m relatively new to this, my previous field of research was the neuromuscular junction Link to comment Share on other sites More sharing options...
Raquel Maria Campos Posted August 13, 2015 Share Posted August 13, 2015 What you used to study in NMJ? Link to comment Share on other sites More sharing options...
erzimmer Posted August 13, 2015 Share Posted August 13, 2015 Dear All, My name is Eduardo, I am a PhD candidate at Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil. I have been working in the interface between biochemistry, behavior and neuroimaging (mostly PET imaging). My main interest is to understand neurodegenerative disorders progression, with particular emphasis in Alzheimer’s disease. I would like to express my gratitude for this opportunity, and it is a pleasure to meet you all. Cheers!! Link to comment Share on other sites More sharing options...
jguarracino Posted August 13, 2015 Share Posted August 13, 2015 I used NMJ as a models to study purinergic signalling Link to comment Share on other sites More sharing options...
janisedalpai Posted August 13, 2015 Share Posted August 13, 2015 Welcome! I´m from POA, but I´m living in SP. Link to comment Share on other sites More sharing options...
Raquel Maria Campos Posted August 13, 2015 Share Posted August 13, 2015 I’m not working with the terapy, it’s another girl from my lab. I’m doing differents types of peripheral lesion, on the sciatic nerve, and observing the changes on synapses on Spinal Cord. I see a reduction os pre-synaptic afferents at motoneurons after injury Link to comment Share on other sites More sharing options...
r_rosas Posted August 13, 2015 Share Posted August 13, 2015 I’m at Universidad Autónoma de San Luis Potosí, at the Laboratorio de Biofísica Molecular, I’m working with effects of ethanol on the cerebellum (effects on the cytoskeleton) but we want to try and do the same experiments using other drugs Link to comment Share on other sites More sharing options...
Raquel Maria Campos Posted August 13, 2015 Share Posted August 13, 2015 Sorry already ask you something, but I’m having huge trouble trying to double staining NMJ with some pre-synaptic marker. I already tried sinaptophysin and Neurofilament 200, do you ever did some stining like that? pre and post synaptic elements? Link to comment Share on other sites More sharing options...
CelesteR Posted August 13, 2015 Share Posted August 13, 2015 If you need a protocol or some advice, I’m working with primary cultures of astrocytes and cerebellar granular cells from rat Link to comment Share on other sites More sharing options...
janisedalpai Posted August 13, 2015 Share Posted August 13, 2015 That is cool! Thanks a lot! I really want to learn mnore about this! Link to comment Share on other sites More sharing options...
r_rosas Posted August 13, 2015 Share Posted August 13, 2015 You are very kind, I would like to read you protocol, thank you! Link to comment Share on other sites More sharing options...
jguarracino Posted August 13, 2015 Share Posted August 13, 2015 I performed a few immunofluoresecence assays but I never tried to mark presynaptic structures other than the particular purinergic receptors I was studying at the time. For post synaptic labelling I used bungarotoxin coupled to rhodamine. Link to comment Share on other sites More sharing options...
Manuel Solino Posted August 13, 2015 Share Posted August 13, 2015 Hello everyone, I’m Manuel Soliño and MD from the University of Buenos Aires in Argentina. I’m working as a PhD fellow on a model of Light induced retinal degeneration in rats. I study the role of endocannabinoid system and the adenosine mediated transmission and their potential as neuroprotectors in the retina. I’m very interested in neuronal degeneration and neuroprotection. I hope that these topics are discussed in the programme. Link to comment Share on other sites More sharing options...
Raquel Maria Campos Posted August 13, 2015 Share Posted August 13, 2015 just like I do, but since I also study regeneration and reinervation after peripheral nervous system injury, I try to stain both strutures Link to comment Share on other sites More sharing options...
janisedalpai Posted August 13, 2015 Share Posted August 13, 2015 That is cool! Thanks a lot! I really want to learn more about this! Would you send your protocol over email? Link to comment Share on other sites More sharing options...
Raquel Maria Campos Posted August 13, 2015 Share Posted August 13, 2015 In my lab there is a group of stydy on endocannabinoids as well. Not my field, but they are working with cannabinoids and neurogenisis Link to comment Share on other sites More sharing options...
jguarracino Posted August 13, 2015 Share Posted August 13, 2015 Truth be told, I had the synaptophising primary antibody but I could never buy a blue secondary antibody to perform a triple labeling. Consider that synaptophising binds to intracellular structures, have you tried treating your tissue slides with some kind of detergent such as triton-x? Link to comment Share on other sites More sharing options...
Raquel Maria Campos Posted August 13, 2015 Share Posted August 13, 2015 yes, but when I do that I wash out purinergic receptor Link to comment Share on other sites More sharing options...
CelesteR Posted August 13, 2015 Share Posted August 13, 2015 I can send you the protocols, and the citation. Link to comment Share on other sites More sharing options...
jguarracino Posted August 13, 2015 Share Posted August 13, 2015 Itś weird to find an MD doing science in Argentina Link to comment Share on other sites More sharing options...
janisedalpai Posted August 13, 2015 Share Posted August 13, 2015 That would be great! janisedalpai@gmail.com Thanks a lot! Link to comment Share on other sites More sharing options...
ccheatham Posted August 13, 2015 Share Posted August 13, 2015 There is an LATP Associate certificate for completing the program. Link to comment Share on other sites More sharing options...
erzimmer Posted August 13, 2015 Share Posted August 13, 2015 Hello Eva, We are working in the same field. I am very interested in glutamatergic abnormalities in Alzheimer’s disease. Are you working with any specific model? Link to comment Share on other sites More sharing options...
gregoryjquirk Posted August 13, 2015 Author Share Posted August 13, 2015 Yes Edgar. If you tune in to the majority of LATP webcasts, and participate in NeuroOnline (as you are now), you will receive a signed certificate of completion for the LATP. Link to comment Share on other sites More sharing options...
gregoryjquirk Posted August 13, 2015 Author Share Posted August 13, 2015 De nada. Suerte con el curso… Link to comment Share on other sites More sharing options...
janisedalpai Posted August 13, 2015 Share Posted August 13, 2015 Can we sugest topic to be discussed? I would like to learn more about cell culture and hipocampus neurogenesis Link to comment Share on other sites More sharing options...
janjoel Posted August 13, 2015 Share Posted August 13, 2015 Dr. Quirk in your work you’re seeing the interaction between infralimbic area and the amygdala area observing the avoidance behavior and fear, is this process similar in humans? Link to comment Share on other sites More sharing options...
Manuel Solino Posted August 13, 2015 Share Posted August 13, 2015 We are not alone Regarding the double staining have you tried a sequential incubation of the primary antibodies? You can try first an incubation with a small dose detergent for sinaptophysin or Neurofilament 200 and then incubate with the antibody against the purinergic receptor of choice after having removed the detergent from the tissue. Link to comment Share on other sites More sharing options...
jguarracino Posted August 13, 2015 Share Posted August 13, 2015 Sorry, I can’t really help you with that. Check out this article, they perform triple labelling at the NMJ. J Anat. 2014 Jul;225(1):109-17. doi: 10.1111/joa.12188. Epub 2014 Apr 23. Link to comment Share on other sites More sharing options...
eva_ramos Posted August 13, 2015 Share Posted August 13, 2015 Hi Eduardo! I use the double transgenic APPsw/PS1 Link to comment Share on other sites More sharing options...
eva_ramos Posted August 13, 2015 Share Posted August 13, 2015 but since now the most part of my project is “in vitro”, the next year I want to begin with behaviour…and you? Link to comment Share on other sites More sharing options...
Maria A. González-González Posted August 13, 2015 Share Posted August 13, 2015 HELLO EVERYBODY!! I´m Ale González from Querétaro, México,I´m in the last year of the PhD, I´m working with cerebellum, I´m interested specifically in ependimal glial cells, neuronal circuits and subventricular zones, I´m in the Molecular and Celular Neurobiology in UNAM, Querétaro. Link to comment Share on other sites More sharing options...
erzimmer Posted August 13, 2015 Share Posted August 13, 2015 Hey Eva, Thanks for replying. I have worked with the McGill-Thy-APP rat. It is one of the first models using rats as background. Now I am working with the infusion of ABeta oligomers in rats and mice. Link to comment Share on other sites More sharing options...
janjoel Posted August 13, 2015 Share Posted August 13, 2015 I’m working with humans helping them to reach a better academic performance, I like these theme and I want to know more about that. It would be phenomenal if they argue it. Link to comment Share on other sites More sharing options...
r_rosas Posted August 13, 2015 Share Posted August 13, 2015 Hi Ale! I’m from San Luis Potosí and I’m working with cerebellum too! Link to comment Share on other sites More sharing options...
luci_danielski Posted August 13, 2015 Share Posted August 13, 2015 Hello! I’m Lucinéia Danielski,please call me Luci. I’m a master student in Universidade do Sul de Santa Catarina, in Santa Catarina. My lab. works whit encephalopathy associated sepsis in animal models. Link to comment Share on other sites More sharing options...
janisedalpai Posted August 13, 2015 Share Posted August 13, 2015 Wow! Interesting subject! Do you know if these animals can have seizures? Link to comment Share on other sites More sharing options...
luci_danielski Posted August 13, 2015 Share Posted August 13, 2015 I for one am torn between the pre frontal and hippocampus. Link to comment Share on other sites More sharing options...
Maria A. González-González Posted August 13, 2015 Share Posted August 13, 2015 Hello Rebeca!! awesome! what are you loofing for in cerebellum? Link to comment Share on other sites More sharing options...
gregoryjquirk Posted August 13, 2015 Author Share Posted August 13, 2015 OK Everyone. Raul Paredes and I are signing off, but we encourage you all to continue this stimulating dialogue after the live part concludes, which is the while point of LATP. Best of luck with the course!! Link to comment Share on other sites More sharing options...
r_rosas Posted August 13, 2015 Share Posted August 13, 2015 Changes in the cytoskeleton after stimulation with some drugs, I’m also in my last year, who are you working with? Link to comment Share on other sites More sharing options...
janisedalpai Posted August 13, 2015 Share Posted August 13, 2015 Thanks! I´m looking forward to hearing from you again! Link to comment Share on other sites More sharing options...
jguarracino Posted August 13, 2015 Share Posted August 13, 2015 I’m signing off, too. Bye Link to comment Share on other sites More sharing options...
Maria A. González-González Posted August 13, 2015 Share Posted August 13, 2015 I´m studying peri and subventricular cells in cerebellum, I use strategies as electron microscopy, immunjofluorescence, neuronal tracers and some adenovirals strategies in order to describe the structure and ultrastructure of some specyfic cells there, Is there anybody working with some similar? Link to comment Share on other sites More sharing options...
janisedalpai Posted August 13, 2015 Share Posted August 13, 2015 Me too! Bye everybody! It was very nice to talk to you all. Hoping to talk to you soon! Link to comment Share on other sites More sharing options...
luci_danielski Posted August 13, 2015 Share Posted August 13, 2015 Yes, I’ts very interesting! We do not check any occurrence, although there is cognitive and short and long-term memory damage. And my work is related in neuroinflammation caused by sepsis. Link to comment Share on other sites More sharing options...
alirriba Posted August 13, 2015 Share Posted August 13, 2015 I’m working with the association between obesity induced by fat diet (45%)and panic responses. I found some changes in GABA tonus into DMH in obese rats. And now I’m looking for possible panic like responses in obese rats, mainly cardiovascular and behavioral responses. Link to comment Share on other sites More sharing options...
Maria A. González-González Posted August 13, 2015 Share Posted August 13, 2015 It sounds great Rebeca I hope we could talk over this for this way Link to comment Share on other sites More sharing options...
r_rosas Posted August 13, 2015 Share Posted August 13, 2015 Sure! I hope we can keep in touch Link to comment Share on other sites More sharing options...
Manuel Solino Posted August 13, 2015 Share Posted August 13, 2015 Bye everybody Have a good one! Link to comment Share on other sites More sharing options...
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