scott_owen Posted August 16, 2018 Share Posted August 16, 2018 I’m not totally clear on the design of your experiment. Are you transplantinc cells? Or trying to use opsins to manipulate the activity of a class of neurons already in the brain? We typically do a channelrhodopsin virus injection at the same time that we do the fiber implant, so it’s all done in a single surgery. If you need a cannula, you could do a cannula implant, inject virus through the cannula, then use a fiber threaded down through the cannula to do fiber-optic stimulation. Threading the fiber through the cannula is not a surgery and can be done with the awake animal immediately before a behavioral session. This is much trickier than the more standard optical fiber/ferrule approach, though, because optical fibers can break off in the cannula if the animal twitches, and when that happens there is no recourse. Link to comment Share on other sites More sharing options...
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