Azam Asgarihafshejani is a post-doctoral fellow at faculty of medicine, department of neurosciences, University de Montreal, QC, Canada. She previously was a post-doctoral fellow at biology department, University of Victoria, BC, Canada. Asgarihafshejani’s main research interests lie in Synaptic plasticity and Synaptic transmission. She received her undergraduate degree in Biology from Isfahan University, Isfahan. Iran, her master degree in Animal Physiology from Isfahan University and her PhD in Physiology from Tarbiat Modares University, Tehran, Iran. She completed her first postdoctoral training at University of Victoria, BC, Canada.
Date and Time that I will be available to answer questions: Tuesday Oct 22, 2019 1:00 PM - 5:00 PM
In my project, We develop optogenetic protocols to selectively activate synaptic inputs from CA1 pyramidal cells onto somatostatin interneurons (SOM-INs) using ChR2, and induce long-term potentiation of EPSPs recorded by patch clamp electrodes in slices. We developed the optogenetic stimulation methods using the Polygon Pattern Illumination system. We characterized the optogenetically induced LTP in SOM-INs and showed it was dependent on mGluR1a and mTORC1 signaling. Moreover, We used field potential recordings in hippocampal slices to demonstrate that optogenetically-induced SOM-IN LTP regulates the two output targets of SOMINs: the Schaffer collateral and temporo-ammonic pathways onto CA1 pyramidal cells. Thus, We found that optogenetic activation of CA1 pyramidal inputs is sufficient to induce LTP of SOM-IN afferent synapses, and that long-term plasticity of these inputs, in turn, regulates metaplasticity of CA1 pyramidal cell Schaffer collateral and temporo-ammonic pathways.