xinnanw Posted July 18, 2017 Share Posted July 18, 2017 you are welcome! there are reports showing altered mitochondrial distribution in stroke models, but we need to know more whether the redistribution is beneficial or detrimental Link to comment Share on other sites More sharing options...
heidi.mcbride Posted July 18, 2017 Share Posted July 18, 2017 Good question. Doug Wallace’s lab has lovely EM images of mitochondria docked together where their cristae line up perfectly in heart muscle. They predicted that there would be junction like machineries to contact them and facilitate metabolic flux. And I recall at least one study seeing a connexin at mitochondria. I think it was connexin 43. Link to comment Share on other sites More sharing options...
eas3 Posted July 18, 2017 Share Posted July 18, 2017 s it easier to edit the mitochondrial genome? People have used zinc finger nucleases and restriction enzymes, but CRISPR cannot be done right now. Could we put nuclear genes in here that are either expressed in the mitochondria normally but are deficient or any old nuclear gene that is deficient Yes, this is called allotopic expression and was done many years ago. First stage in gene editing? Link to comment Share on other sites More sharing options...
gomezmoljfmedcol Posted July 18, 2017 Share Posted July 18, 2017 how big a mitoc can be? and their most likely shape is…? sorry if too many questions List item Link to comment Share on other sites More sharing options...
heidi.mcbride Posted July 18, 2017 Share Posted July 18, 2017 The causes of mitochondrial dysfunction can be many! From direct mendelian mutations to somatic drift in mtDNA mutations within tissues. Epigenetic work in mother mice fed different diets have also been shown to change mitochondrial behavior in the pups. But there are no drugs for mitochondrial disease or dysfunction that really work yet. In acute conditions of mito disease they can take CoQ10, but really these are not very useful. Lots still to be done! Link to comment Share on other sites More sharing options...
stebbin2 Posted July 18, 2017 Share Posted July 18, 2017 are there limitations to doing this in a living human? Link to comment Share on other sites More sharing options...
eas3 Posted July 18, 2017 Share Posted July 18, 2017 Connexins 32 (Fowler (2013) J Proteome Res 12:2597) and 43 (Boengler (2012) J Cell Mol Med 16:1649) have been found in mitos. Link to comment Share on other sites More sharing options...
tmhoangt Posted July 18, 2017 Share Posted July 18, 2017 what is the potential drug delivery mechanisms that can reach and affect mito? Link to comment Share on other sites More sharing options...
heidi.mcbride Posted July 18, 2017 Share Posted July 18, 2017 The shape of mitochondria can vary between tissues, as well as the electron densities within the matrix and inner membrane. So it’s a broad question to answer. But once you have a sense of the mito profiles in your tissue of interest, you generally look for cristae aberrations, sometimes you’ll see whorls of membrane, or big white spaces in the intermembrane space, revealing cristae remodelling. You can also sometimes see them contacting lysosomes, and the mitochondrial regions that lie against the lysosome can have very aberrant cristae. The best answer is just to look at loads of EM! Link to comment Share on other sites More sharing options...
eas3 Posted July 18, 2017 Share Posted July 18, 2017 Yes, not the least of which are ethical. Link to comment Share on other sites More sharing options...
frances.johnson Posted July 18, 2017 Share Posted July 18, 2017 Do you have any insights into the role of the DISC-1 gene in mitochondrial transport in neurons? Link to comment Share on other sites More sharing options...
xinnanw Posted July 18, 2017 Share Posted July 18, 2017 the size of mito varies from tissue to tissue, the diameter of a mito can be anywhere from a few hundred nm (like in synapses) to a few micros (in muscles and sperm). Link to comment Share on other sites More sharing options...
xinnanw Posted July 18, 2017 Share Posted July 18, 2017 there are two papers showing DISC1 affects mito motility, probably via the Miro/milton complex Link to comment Share on other sites More sharing options...
gomezmoljfmedcol Posted July 18, 2017 Share Posted July 18, 2017 does the ER has a membrane potential? some says no…and what about the electromot force that generate the outer membrane potential…is intrinsic or from the inner membrane (according to Dr V Lameshko)? Link to comment Share on other sites More sharing options...
heidi.mcbride Posted July 18, 2017 Share Posted July 18, 2017 Certainly it has been shown many years ago by people like Gyorgy Hajnoscky that calcium release from the ER leads to depolarization in the mitochondria. When he added a bit of IP3 to the side of a long myotube he saw a wave of calcium loss from ER that followed the loss of TMRE stain in mitos. That was about 2005 and was beautiful. So yes, certainly the oscillations can be coupled. Just not so far directly with the frequency of neurotransmitter release. Link to comment Share on other sites More sharing options...
gomezmoljfmedcol Posted July 18, 2017 Share Posted July 18, 2017 thank you, very nice you all to answer!!! Link to comment Share on other sites More sharing options...
eas3 Posted July 18, 2017 Share Posted July 18, 2017 Some compounds can enter mitos passively, if they are not too hydrophilic. There are any number of transporters in mitos, some of which might bring in small molecules (e.g. pyruvate) Link to comment Share on other sites More sharing options...
eas3 Posted July 18, 2017 Share Posted July 18, 2017 I think that the field pretty much agrees that there is no membrane potential across the MOM. Link to comment Share on other sites More sharing options...
gomezmoljfmedcol Posted July 18, 2017 Share Posted July 18, 2017 ok…currents from the inner membrane can go throug the outer…and cause some potential drop or not? Link to comment Share on other sites More sharing options...
eas3 Posted July 18, 2017 Share Posted July 18, 2017 Not that I’m aware of Link to comment Share on other sites More sharing options...
heidi.mcbride Posted July 18, 2017 Share Posted July 18, 2017 So we are losing this line now! Thank you all for listening and participating. It was a fun session and I hope we have inspired you to solve all things mitochondrial! Best Heidi Link to comment Share on other sites More sharing options...
gomezmoljfmedcol Posted July 18, 2017 Share Posted July 18, 2017 sure thanks more to all of you!! great!!! Link to comment Share on other sites More sharing options...
Recommended Posts
Please sign in to comment
You will be able to leave a comment after signing in